Multiple interfaces between a serine recombinase and an enhancer control site-specific DNA inversion.

Serine recombinases are often tightly controlled by elaborate, topologically-defined, nucleoprotein complexes. Hin is a member of the DNA invertase subclass of serine recombinases that are regulated by a remote recombinational enhancer element containing two binding sites for the protein Fis. Two Hin dimers bound to specific recombination sites associate with the Fis-bound enhancer by DNA looping where they are remodeled into a synaptic tetramer competent for DNA chemistry and exchange. Here we show that the flexible beta-hairpin arms of the Fis dimers contact the DNA binding domain of one subunit of each Hin dimer. These contacts sandwich the Hin dimers to promote remodeling into the tetramer. A basic region on the Hin catalytic domain then contacts enhancer DNA to complete assembly of the active Hin tetramer. Our results reveal how the enhancer generates the recombination complex that specifies DNA inversion and regulates DNA exchange by the subunit rotation mechanism. DOI:http://dx.doi.org/10.7554/eLife.01211.001

Magnetic and humidity sensing properties of nanostructured Cu[x]Co[1-x]Fe2O4 synthesized by auto combustion technique

Magnetic nanomaterials (23-43 nm) of Cu$_x$Co$_{1-x}$Fe$_2$O$_4$\ (x = 0.0, 0.5 and 1.0) were synthesized by auto combustion method. The crystallite sizes of these materials were calculated from X-ray diffraction peaks. The band observed in Fourier transform infrared spectrum near 575 cm$^{-1}$ in these samples confirm the presence of ferrite phase. Conductivity measurement shows the thermal hysteresis and demonstrates the knee points at 475$^o$C, 525$^o$C and 500$^o$C for copper ferrite, cobalt ferrite and copper-cobalt mixed ferrite respectively. The hystersis M-H loops for these materials were traced using the Vibrating Sample Magnetometer (VSM) and indicate a significant increase in the saturation magnetization (M$_s$) and remanence (M$_r$) due to the substitution of Cu$^{2+}$ ions in cobalt ferrite, while the intrinsic coercivity (H$_c$) was decreasing. Among these ferrites, copper ferrite exhibits highest sensitivity for humidity.Comment: 12 pages, 7 figure

Stabilisation of Red Phosphorus to Prevent Moisture Absorptionand Suppression of Phosphine Release

Red phosphorus (RP) is an essential ingredient to generate smoke for multi-spectralobscuration and is receiving wide attention throughout the world for military applications.However, oxidation of phosphorus occurs in moist air resulting in the formation of variousphosphorus acids and subsequently the evolution of the toxic gas, phosphine (PH3), even inthe sealed systems. Entrapped moisture leads to deterioration of the stores. The commercial-RPat ambient temperature and 95 per cent  RH conditions indicates 15 per cent  moisture absorptionand 13 per cent acidity development with the generation of more than 100 ppm of phosphinegas. Efforts were made to overcome this problem by suppressing acid formation. The technologyadopted was: (i) use of stabiliser, and (ii) doping stabiliser-coated particles with polymericsubstance. The aim to use metal oxide as stabiliser was to neutralise the phosphorus acids,which are catalysing the phosphine generation. MgO due to its basic nature seems to be fasterthan amphoteric Al2O3, in removing the acid from site of reaction, thus suppressing the phosphinerelease effectively. Though MgCO3 as stabiliser suppresses moisture absorption and aciditydevelopment, phosphine release is not controlled effectively. Study of RP coated with thesematerials independently and monitoring them at different RH conditions indicated superiorityof MgO in suppressing acidity and phosphine formation

FPGA IMPLEMENTATION OF RED ALGORITHM FOR HIGH SPEED PUPIL ISOLATION

Iris recognition is an automated method of biometric identification that uses mathematical pattern-recognition techniques on video images of the irises of an individual’s eyes, whose complex random patterns are unique and can be seen from some distance. Modern iris recognition algorithms can be computationally intensive, yet are designed for traditional sequential processing elements, such as a personal computer. However, a parallel processing alternative using Field Programmable Gate Array offers an opportunity to speed up iris recognition. Within the means of this project, iris template generation with directional filtering, which is a computationally expensive, yet parallel portion of a modern iris recognition algorithm, is parallelized on an FPGA system. An algorithm that is both accurate and fast in a hardware design that is small and transportable are crucial to the implementation of this tool. As part of an ongoing effort to meet these criteria, this method improves a iris recognition algorithm, namely pupil isolation. A significant speed-up of pupil isolation by implementing this portion of the algorithm on a Field Programmable Gate Array

Developing procedures for assessment of ecological status of Indian River basins in the context of environmental water requirements

River basins / Ecology / Indicators / Environmental flows / Environmental management / Habitats / Biota / Fish / Ecosystems / India / Krishna River Basin / Chauvery River Basin / Narmada River Basin / Periyar River Basin / Ganga River Basin

Nitramine Double Base Propellants

This review paper broadly covers the studies conducted on nitramine double base (DB) propellants, particularly in the field of formulations, evaluation, catalysis and combustion mechanism . Addition of RDX and HMX in double base matrix shows relatively low burn rates and high paper index values. Further, the burn rate of this class of propellants enhances in the presence of energetic binders/plasticisers like glycidyl azide polymer. This paper also discusses the combustion mechanism of HMX/RDX-based DB propellants, especially in the presence of catalytic salts. As scanty data is available on extruded nitramine DB propellants, further work is needed in the field of formulation as well as evaluation with a view to generate exhaustive data

Constraining the Variability and Binary Fraction of Galactic Center Young Stars

We present constraints on the variability and binarity of young stars in the central 10 arcseconds (~0.4 pc) of the Milky Way Galactic Center (GC) using Keck Adaptive Optics data over a 12 year baseline. Given our experiment's photometric uncertainties, at least 36% of our sample's known early-type stars are variable. We identified eclipsing binary systems by searching for periodic variability. In our sample of spectroscopically confirmed and likely early-type stars, we detected the two previously discovered GC eclipsing binary systems. We derived the likely binary fraction of main sequence, early-type stars at the GC via Monte Carlo simulations of eclipsing binary systems, and find that it is at least 32% with 90% confidence.Comment: Accepted for publication in Proceedings of IAU Symposium 322: The Multi-Messenger Astrophysics of the Galactic Centre, 2 pages, 1 figur

Thin-thick surface phase coexistence and boundary tension of the square-well fluid on a weak attractive surface

Prewetting transition is studied for the square-well fluid of attractive-well diameter ??ff ff =1.5 in the presence of a homogeneous surface modeled by the square-well potential of attractive well from 0.8 ff to 1.8 ff. We investigate surface phase coexistence of thin-thick film transition using grand-canonical transition matrix Monte Carlo (GC-TMMC) and histogram reweighting techniques. Molecular dynamics (MD) and GC-TMMC are utilized to predict the properties of the fluid for various surface fluid affinities. Occurrences of prewetting transition with the variation of surface affinity are observed for a domain of reduced temperature from T* =0.62 to 0.75. We have used MD and GC-TMMC+finite size scaling (FSS) simulations to calculate the boundary tension as a function of temperature as well as surface affinity. Boundary tensions via MD and GC-TMMC+FSS methods are in good agreement. The boundary tension increases with the decrease of wall-fluid affinity. Prewetting critical properties are calculated using rectilinear diameter approach and scaling analysis. We found that critical temperature and density increase with the decrease of wall-fluid affinity.open101